anti gfap  (Cell Signaling Technology Inc)

Journal: International Journal of Molecular Sciences

Article Title: GABA-Induced Exosomes Improve Memory Impairment in Aged Mice

doi: 10.3390/ijms27062519

Figure Lengend Snippet: Effects of exosomes derived from GABA-fed mice on hippocampus in aged mice. ( a ) Brain sections (dentate gyrus, DG) were incubated with primary antibody for anti-Iba1, anti-GFAP, anti-p21 and anti-β-galactosidase, and stained with Alexa Fluor 555. After staining with Vestashield mounting medium, the tissue samples were observed under a fluorescence microscope. A 50 μm scale bar is shown in the lower right corner of each photograph. ( b ) Number of Iba1 + cells per area and relative area of GFAP + cells, p21 + cells and SA-β-Gal + cells in DG, CA3 and CA1 are shown as bar graphs (* p < 0.05; ** p < 0.01; *** p < 0.001 vs. Adult mice; # p < 0.05; ## p < 0.01; ### p < 0.001 vs. Aged (Ctrl-Exo) mice; data are represented as means ± SEM, n = 10). GABA: Gamma-aminobutyric acid.

Article Snippet: Brain sections were incubated with primary antibodies for anti-Iba1 (#17197, Cell Signaling Technology, Danvers, MA, USA), anti-GFAP (#80788, Cell Signaling Technology), anti-p21 (#2974, Cell Signaling Technology), and anti-β Galactosidase (15518-1-AP, Proteintech, Rosemont, IL, USA), and subsequently with secondary antibodies (Alexa Fluor 555 anti-rabbit IgG, Thermo Fischer Scientific) [ ].

Techniques: Derivative Assay, Incubation, Staining, Fluorescence, Microscopy

Journal: Acta Pharmaceutica Sinica. B

Article Title: Reversibly unlocking the blood–brain barrier: A study on microtubule dynamics modulation using gold nanoparticle-modified reduced graphene oxide

doi: 10.1016/j.apsb.2025.12.015

Figure Lengend Snippet: The BBB-reversible opening effects by Au-rGO in vivo . (A) Schematic representation of Au-rGO exposure and the Evans blue permeability test in vivo . (B) Brain tissue staining with Evans blue from mice intravenously exposed to Au-rGO at different doses. (C) Brain tissue staining with Evans blue from mice intravenously exposed to Au-rGO at a dose of 4 mg/kg body weight for various durations ( n = 3). (D) Vibratome sections of brain tissue stained with horseradish peroxidase from mice exposed to Au-rGO at a dose of 4 mg/kg body weight for different time points ranging from 30 min to 7 days. (E) Immunoblot analysis of VE-cadherin and Claudin-5 in brain tissue from mice exposed to Au-rGO at a dose of 4 mg/kg body weight for 1, 2, 6 h, 1 day, and 7 days. (F) Top-to-bottom continuous brain sections from mice exposed to Au-rGO at a dose of 4 mg/kg body weight for 2 h. (G) Measurement of Au content as an indicator of Au-rGO retention in brain tissue using the inductively coupled plasma mass spectrometry (ICP-MS). (H) Immunoblot analysis of glial fibrillary acidic protein (GFAP) in brain tissue from mice exposed to Au-rGO at a dose of 4 mg/kg body weight for 1, 2, 6 h, 1 day, and 7 days. (I) IF staining of GFAP (green) reveals reactive astrocytes around brain vessels, with CD31 (red) used to mark brain vessels. Scale bar: 10 μm. (J) IF staining of albumin (red) extravasation around brain vessels, with CD31 (green) used to mark brain vessels. Scale bar: 50 μm. Data are represented as the mean ± SD ( n = 5 for the rest of the in vivo experiments). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 compared with the control group. # P < 0.05, ## P < 0.01 and ### P < 0.001 compared between the two experiment groups.

Article Snippet: For brain immunofluorescence (IF) staining, CD31 antibody (Proteintech 11265-1-AP (rabbit), IL, USA; Huabio M1511-8 (mouse), Hangzhou, China) was used to mark brain vessels, glial fibrillary acidic protein (GFAP) antibody (Cell Signaling Technology 80788, MA, USA) was used to mark reactive astrocytes around brain vessels, and Albumin antibody (Proteintech 16475-1-AP, IL, USA) was used to mark Albumin extravasation around brain vessels.

Techniques: In Vivo, Permeability, Staining, Western Blot, Clinical Proteomics, Mass Spectrometry, Control